154. Adenosine Diphosphate

Nomenclature

CAS number: 58-64-0
Adenosine 5′-(trihydrogen diphosphate); ADP; adenosine 5′-pyrophosphoric acid; 5′-adenylphosphoric acid; adenosinediphosphoric acid.
C10H15N5O10P2; mol wt 427.20.
C 28.11%, H 3.54%, N 16.39%, O 37.45%, P 14.50%.

Description and references

Formed from ATP in the muscle by the enzyme adenosinetriphosphatase upon stimulation of the muscle unless hydrolysis is prevented by injection of magnesium sulfate. Prepd by hydrolysis of ATP: LePage, Biochem. Prep. I, 1 (1949). Synthesis: Chambers et al., J. Am. Chem. Soc. 82, 970 (1960). See also the ref and data under Adenosine Triphosphate.

Chemical structure

Derivative

Barium salt.
Ba3(C10H12N5O10P2)2; mol wt 1260.34.
Ba 32.69%, C 19.06%, H 1.92%, N 11.11%, O 25.39%, P 9.83%.

Properties

The purity of the prepns can be checked by analyses for nitrogen, ribose (orcinol reaction), total phosphorus, easily hydrolyzable phosphorus, and inorganic phosphorus. ADP should give a ratio of 2:1 for total organic phosphorus to easily hydrolyzable phosphorus. uv max (pH 7.0): 259 nm (aM 15.4 × 103).