218. Alcian Blue

Nomenclature

CAS number: 12040-44-7
C.I. Ingrain Blue 1; C.I. 74240; alcian blue 8GX; michrome no. 24.

Description and references

When R = toluyl, the mol wt is about 1300. Discovered by Haddock and Wood in 1944: Haddock, Research 1, 685 (1948); GB 586340 and GB 587636 (both to ICI). During dyeing the X groups split off. Prepn: Colour Index vol. 4 (3rd ed.) p 4620.

Chemical structure

Properties

Greenish-black crystals with metallic sheen. When R = toluyl the solubility in water at 20° is about 9.5% w/w giving bright greenish-blue solutions. Soly in absolute ethanol about 6.0, Cellosolve 6.0, ethylene glycol 3.25. Practically insoluble in xylene.

Use

Gelling agent for lubricating fluids. As bacterial stain. To dye histiocytes and fibroblasts in vivo: McManus, Bailie, Fed. Proc. 22, no. 2, part I, p 190 (March-April 1963).