2055. CHES

Nomenclature

CAS number: 103-47-9
2-(Cyclohexylamino)ethanesulfonic acid; N-cyclohexyltaurine.
C8H17NO3S; mol wt 207.29.
C 46.35%, H 8.27%, N 6.76%, O 23.16%, S 15.47%.

Description and references

Zwitterionic N-substituted aminosulfonic acid in the style of the “Good” buffers; active in the pH range 8.6-10.0. Synthesis: A. Champseix et al., Bull. Soc. Chim. Fr. 1985, 463. Effects of freezing on buffering: D. L. Williams-Smith et al., Biochem. J. 167, 593 (1977). Effects on Lowry protein assay: C. Cookson, Anal. Biochem. 88, 340 (1978). Dissociation constants and buffering capacity: M. J. Taylor, Y. Pignat, Cryobiology 19, 99 (1982). Use as buffer: M. G. N. Hartmanis, T. C. Stadtman, Proc. Natl. Acad. Sci. USA 84, 76 (1987); C. Engstrand et al., Biochim. Biophys. Acta 1122, 321 (1992).

Chemical structure

Properties

Crystals, mp 320°. pKa in water (25°) 9.27 ±0.01; in 20% (w/w) DMSO (25°) 9.10, (0°) 9.76, (-5.5°) 10.01; in 30% (w/w) DMSO (25°) 9.11, (0°) 9.89, (-12°) 10.27.

Use

Biological buffer.