2274. Ciliary Neurotrophic Factor

Nomenclature

CNTF.

Description and references

Neurotrophic factor unrelated to the neurotrophins, q.v. Cytosolic peptide produced by Schwann cells in the peripheral nervous system and by astrocytes in the CNS; not expressed by target tissues. Promotes survival of a broad range of neurons and enhances differentiation of neurons and astrocytes. Initial identification as in vitro survival factor for chick ciliary neurons: R. Adler et al., Science 204, 1434 (1979). Purification from chick eye: G. Barbin et al., J. Neurochem. 43, 1468 (1984); from rat sciatic nerve: M. Manthorpe et al., Brain Res. 367, 282 (1986). Molecular cloning of rat CNTF: K. A. St"ockli et al., Nature 342, 920 (1989); of rabbit CNTF: L.-F. H. Lin et al., Science 246, 1023 (1989); of human CNTF: A. Lam et al., Gene 102, 271 (1991); A. Negro et al., Eur. J. Biochem. 201, 289 (1991). Effect on motoneuron survival and proposed role as a “lesion factor” released in pathophysiological conditions: M. Sendtner et al., J. Cell Sci. Suppl. 15, 103 (1991). Review of bioactivity and structural relationship with hematopoietic cytokines: N. Y. Ip, G. D. Yancopoulos, Prog. Growth Factor Res. 4, 139-155 (1992). Review of CNTF receptor: S. Davis, G. D. Yancopoulos, Curr. Opin. Neurobiol. 5, 281-285 (1993). Review: M. Manthorpe et al. in Neurotrophic Factors, S. E. Loughlin, J. H. Fallon, Eds. (Academic Press, San Diego, 1993) pp 443-473.

Derivative

Human CNTF.

Nomenclature

CAS number: 133423-39-9

Properties

200 Amino acid peptide. mol wt ≈23,000 Da. pI ≈6.0. Exhibits ≈83% amino acid homology with rat CNTF; ≈87% with rabbit CNTF.