2679. Cy 5
Nomenclature
CAS number: 146368-15-2
1-[6-[-(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-2-[5-[1-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene]-1,3-pentadienyl]-3,3-dimethyl-5-sulfo-3H-indolium inner salt. C45H52N4O14S2; mol wt 937.04.
C 57.68%, H 5.59%, N 5.98%, O 23.90%, S 6.84%.
Description and references
Indocyanine dye with high fluorescence which is easily coupled to proteins. Named CyX.Y where X is the number of bridge carbons and Y represents a unique dye structure. Prepn: A. S. Waggoner et al., DE 3912046; eidem, US 5268486 (1990, 1993 both to Carnegie-Mellon). Use with fiber optic biosensors: L. A. Tempelman et al., Proc. SPIE Int. Soc. Opt. Eng. 2293, 139 (1994); for antibody/antigen detection: R. M. Wadkins et al., ibid. 2676, 148 (1996). Use in resonance energy transfer: P. R. Selvin et al, J. Am. Chem. Soc. 116, 6029 (1994).
Properties
Water soluble.Derivative
Potassium salt.Properties
Water soluble. Absorbance max 650 nm; emission max 667 nm (ε >200,000). Quantum yield 0.28.Derivative
Cy 5.5.Nomenclature
CAS number: 172777-84-3