4071. Fibrinogen

Nomenclature

CAS number: 9001-32-5
Factor I; Parenogen (Miles).

Description and references

A plasma glycoprotein belonging structurally to the keratin-myosin group. Synthesized and secreted by hepatic parenchymal cells. Present to the extent of 0.3-0.4 g/100 ml in human plasma. Essential to the clotting of blood. Its synthesis is greatly increased during acute inflammatory challenge. The fibrinogen molecule consists of three peptide chains, α (A), β (B), and γ (C), crosslinked by several disulfide bonds. The mol wt of about 400,000 represents a dimeric form of the molecule. Thrombin releases fibrinopeptides A and B from the N-terminal ends of the α and β chains of fibrinogen in the formation of fibrin during coagulation. Because fibrinogen is less sol than other plasma proteins it is readily separated by precipitation with sodium chloride: Florkin, J. Biol. Chem. 87, 629 (1930); or with ammonium sulfate: Nanninga, Arch. Neerl. Physiol. 28, 241 (1946). Prepn from human plasma: Edsall et al., J. Am. Chem. Soc. 69, 2731 (1947). Purification: Cama et al., Naturwissenschaften 48, 574 (1961). End group determination: Lorand, Middlebrook, Science 118, 515 (1953). Structure studies: Schauenstein, Hochenegger, Z. Naturforsch. 8b, 473 (1953); Edsall, J. Polym. Sci. 12, 253 (1954). Reviews: Seegers, Physiol. Rev. 34, 711 (1954); Lorand, Fed. Proc. 24, no. 4, part 1, 784 (1965); several authors, Thromb. Diath. Haemorrh. Suppl. 39 (1970); A. L. Copley, Thromb. Res. 14, 249 (1979). Fibrinogen has been shown to be the receptor for the endogenous lectin (agglutinin) secreted by activated platelets: T. K. Gartner et al., Nature 289, 688 (1981). Review of biosynthesis: G. M. Fuller, D. G. Ritchie, Ann. N.Y. Acad. Sci. 389, 308-322 (1982).

Properties

Sparingly sol in water. Aq solns are viscous. Isoelectric point 5.5. Readily denatured by heating to 56° or higher, and by chemical agents such as salicylaldehyde, naphthoquinone sulfonates, ninhydrin, and alloxan. Small amounts of papain will clot fibrinogen, but larger amounts will digest the clot.

Therapeutic Category

Coagulant (clotting factor).

Keywords

Hemostatic