5485. Light Green SF Yellowish

Nomenclature

CAS number: 5141-20-8
N-Ethyl-N-[4-[[4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl](4-sulfophenyl)methylene]-2,5-cyclohexadien-1-ylidene]-3-sulfobenzenemethanaminium inner salt, disodium salt; C.I. Acid Green 5; ethyl[4-[p-[ethyl(m-sulfobenzyl)amino]-α-(p-sulfophenyl)benzylidene]-2,5-cyclohexadien-1-ylidene](m-sulfobenzyl)ammonium hydroxide inner salt disodium salt; C.I. 42095; FD & C Green No. 2; Lissamine Green SF (Zeneca).
C37H34N2Na2O9S3; mol wt 792.85.
C 56.05%, H 4.32%, N 3.53%, Na 5.80%, O 18.16%, S 12.13%.

Description and references

Prepn and refs: Colour Index vol. 4 (3rd ed., 1971) p 4385. Biological use: H. J. Conn's Biological Stains, R. D. Lillie, Ed. (Williams & Wilkins, Baltimore, 9th ed., 1977) pp 257-258, 582. Toxicity data: F. C. Lu, A. Lavalle, Can. Pharm. J. 97, 30 (1964). Chronic toxicity study: W. H. Hansen et al., Food Cosmet. Toxicol. 4, 389 (1966).

Chemical structure

Properties

A reddish-brown powder. Sol in water to a green soln which turns yellowish-brown with HCl and then gradually fades. Addition of NaOH almost completely decolorizes the soln yielding a dull violet ppt. LD50 orally in rats: >2 g/kg (Lu, Lavalle).

Use

As dye, biological stain.