5594. Lucifer Yellow(s)

Description and references

Water soluble fluorescent dyes. Description of properties and potential uses: W. W. Stewart, Cell 14, 741 (1978). Prepn: idem, J. Am. Chem. Soc. 103, 7615 (1981). Use in flux kinetics: C. L. Bowman, H. Tedeschi, Biochim. Biophys. Acta 731, 261 (1983); in intercellular communications: M. H. El-Fouly et al., Exp. Cell Res. 168, 422 (1987); as covalent marker: K. M. Lowe, R. E. McCarty, Biochemistry 37, 2507 (1998).

Chemical structure

Derivative

Lucifer Yellow CH.

Nomenclature

CAS number: 67769-47-5
6-Amino-2-[(hydrazinocarbonyl)amino]-2,3-dihydro-1,3-dioxo-1H-benz[de]isoquinoline-5,8-disulfonic acid dilithium salt; lucifer yellow carbohydrazide; 4-amino-N-[(hydrazinocarbonyl)amino]-2,3-naphthalimide-3,6-disulfonate.
C13H9Li2N5O9S2; mol wt 457.25.
C 34.15%, H 1.98%, Li 3.04%, N 15.32%, O 31.49%, S 14.03%.

Properties

Fluffy orange hygroscopic powder. uv max (water, excitation max): 280, 428 nm (ε 24200, 11900); emission max: 540 nm. Quantum yield: 0.21.

Derivative

Lucifer Yellow VS.

Nomenclature

CAS number: 71231-14-6
6-Amino-2-[3-(ethenylsulfonyl)phenyl]-2,3-dihydro-1,3-dioxo-1H-benz[de]isoquinoline-5,8-disulfonic acid dilithium salt; lucifer yellow vinylsulfone.
C20H12Li2N2O10S3; mol wt 550.39.
C 43.64%, H 2.20%, Li 2.52%, N 5.09%, O 29.07%, S 17.48%.

Properties

Crystals. uv max (water, excitation max): 280, 428 nm (ε 24600, 12200); emission max: 540 nm. Quantum yield: 0.24.

Use

Fluoroprobe for biological tracing.