7542. PMSF

Nomenclature

CAS number: 329-98-6
Benzenemethanesulfonyl fluoride; phenylmethanesulfonyl fluoride; α-toluenesulfonyl fluoride.
C7H7FO2S; mol wt 174.19.
C 48.27%, H 4.05%, F 10.91%, O 18.37%, S 18.41%.

Description and references

Serine and cysteine protease inhibitor. Prepn: W. Davies, J. H. Dick, J. Chem. Soc. 1932, 483; and protease inhibition study: D. E. Fahrney, A. M. Gold, J. Am. Chem. Soc. 85, 997 (1963). Stability studies: G. T. James, Anal. Biochem. 86, 574 (1978). Analgesic effects and toxicity in rodents: C. Pinsky et al., Life Sci. 31, 1193 (1982). Neuropathologic effects in organophosphorus ester-induced delayed neuropathy in hens: C. Massicotte et al., Neurotoxicology 20, 749 (1999).

Chemical structure

Properties

Colorless needles from light petroleum, mp 90-91°. LD50 (24 hr) in mice (mg/kg): 215 ±55 i.p. (Pinsky).

Use

Standard protease inhibitor in biological research; in protein purification to prevent proteolytic degradation.