Antitumor ribonuclease isolated from oocytes and early embryos of Rana pipiens. Single chain protein containing 104 amino acid residues; mol wt ≈12 kDa. Description of cytotoxic activity: Z. Darzynkiewicz et al., Cell Tissue Kinet. 21, 169 (1988). Amino acid sequence and identification as a ribonuclease: W. Ardelt et al., J. Biol. Chem. 266, 245 (1991). Crystallization: S. C. Mosimann et al., Proteins Struct. Funct. Genet. 14, 392 (1992). Mechanism of action: Y. Wu et al., J. Biol. Chem. 268, 10686 (1993). Prepn by recombinant technology: E. Notomista et al., FEBS Lett. 463, 211 (1999). Clinical trial in malignant mesothelioma: S. M. Mikulski et al., J. Clin. Oncol. 20, 274 (2002).
Antineoplastic.
Antineoplastic; Enzymes