8885. Sudan Black B

Nomenclature

CAS number: 4197-25-5
2,3-Dihydro-2,2-dimethyl-6-[[4-(phenylazo)-1-naphthalenyl]azo]-1H-perimidine; C.I. Solvent Black 3; C.I. 26150; SBB; SSB; SBB-II; SSB-II.
C29H24N6; mol wt 456.54.
C 76.29%, H 5.30%, N 18.41%.

Description and references

Lipid dye comprised of two major blue components SBB-I, SBB-II and at least 12 secondary components. SBB-II is defined by the chemical name and CAS registry number cited. Introduced: L. Lison, C. R. Seances Soc. Biol. Ses Fil. 115, 202 (1934). TLC purification and histochemistry: A. G. W. Lansink, Histochemie 16, 68 (1968); and solvent effects: W. M. Frederiks et al., Acta Histochem. Suppl. 24, 259 (1981). Structure elucidation: U. Pfüller et al., Histochemistry 54, 237 (1977). Staining technique: D. A. Nelson, F. R. Davey, “Sudan Black B Staining” in Hematology, W. J. Williams et al., Eds. (McGraw-Hill Publishing Co., New York, 1990) pp 1747-1748. Use as lipid stain: H. N. Subramaniam, K. A. Chaubal, J. Biochem. Biophys. Methods 21, 9 (1990).

Chemical structure

Properties

Basic compound completely decomposed at 180-186°.

Derivative

Sudan Black B I.

Nomenclature

SBB-I; SSB-I; 2,3-dihydro-2,2-dimethyl-4[(4-phenylazo-1-naphthalenyl)-azo]-1H-perimidine.

Properties

Neutral compound, mp 180-186° (dec). Sublimates 140-160° at 103 torr without decompositon.

Use

Biological stain for lipids.