Dimidium Bromide-Sulfan Blue Mixed Solution

Dimidium bromide-sulphan blue mixed solution

Dissolve separately 0.5 g of dimidium bromide and 0.25 g of sulfan blue in 30 mL of a hot mixture of 1 volume of anhydrous ethanol and 9 volumes of water, stir, mix the two solutions, and dilute to 250 mL with the same mixture of solvents. Mix 20 mL of this solution with 20 mL of a 14.0% V/V solution of sulfuric acid previously diluted with about 250 mL of water and dilute to 500 mL with water.

Store protected from light.