Ethylene Oxide Solution

Weigh a quantity of cool ethylene oxide stock solution containing 2.5 mg of ethylene oxide into a cool flask and dilute to 50 g with polyethylene glycol 200 R1. Mix well and dilute 2.5 mg of this solution to 25 mL with polyethylene glycol 200 R1 (5 µg of ethylene oxide per g of solution). Prepare immediately before use.

The solution can be prepared using commercially available reagents instead of ethylene oxide stock solution, making appropriate dilutions.