SDS-PAGE Sample Buffer (Concentrated)

Dissolve 1.89 g of tris(hydroxymethyl)aminomethane, 5.0 g of sodium lauryl sulfate and 50 mg of bromophenol blue in water. Add 25.0 mL of glycerol and dilute to 100 mL with water. Adjust the pH to 6.8 with hydrochloric acid, and dilute to 125 mL with water.