Tris(hydroxymethyl)aminomethane-EDTA Buffer Solution pH 8.4 R1

Dissolve 10.20 g of sodium chloride, 6.10 g of tris(hydroxymethyl)aminomethane, 2.80 g of sodium edetate and 1.00 g of macrogol 6000 or 2.00 g of bovine albumin or of human albumin in 800 mL of water. Adjust to pH 8.4 with hydrochloric acid and dilute to 1.0 L with water.