Appendix XV C. Calcium in Adsorbed Vaccines

All solutions used for this test must be prepared usingwater R.

Determine the calcium by atomic emission spectrometry (2.2.22, Method I). Homogenise the preparation to be examined. To 1.0 mL add 0.2 mL of dilute hydrochloric acid R and dilute to 3.0 mL with water R. Measure the absorbance at 620 nm.