Azo dye. Prepn: Knecht, J. Soc. Dyers Colour. 2, 24 (1886). See also Colour Index vol. 4 (3rd ed., 1971) p 4093. Metabolism: J. L. Radomski, T. J. Mellinger, J. Pharmacol. Exp. Ther. 136, 259 (1962). Use as indicator: N. K. Murty, K. R. Rao, J. Indian Chem. Soc. 53, 532 (1976). Stability studies: A. Motawi, S. El-Gamal, Sci. Pharm. 48, 377 (1980). Effect of pH and concentration on reduction: J. Maslowska, J. Janiak, Zesz. Nauk. Politech. Lodz. 1994, 150. Spectrophotometric analysis: C. C. Blanco et al., Talanta 43, 1019 (1996). Review of carcinogenic risk: IARC Monographs 8, 41-60 (1975).