929. Azure C

Nomenclature

CAS number: 531-57-7
3-Amino-7-(methylamino)phenothiazin-5-ium chloride; 3-imino-7-(methylamino)-3H-phenothiazine hydrochloride; 3-amino-7-methylaminophenazathonium chloride; monomethyldiaminodiphenazothionium chloride; monomethylthionine chloride; C.I. 52002.
C13H12ClN3S; mol wt 277.77.
C 56.21%, H 4.35%, Cl 12.76%, N 15.13%, S 11.54%.

Description and references

Prepn: Holmes, French, Stain Technol. 1, 17 (1926). Review: H. J. Conn's Biological Stains, R. D. Lillie, Ed. (Williams & Wilkins, Baltimore, 9th ed., 1977) pp 419-420, 604. See also: Colour Index vol. 4 (3rd ed., 1971) p 4469.

Chemical structure

Properties

Green glistening crystals or dark green powder. Sol in water (blue soln); sparingly sol in alc. Absorption max: 608-622 nm (3 ml of a soln of 50 mg in 250 ml H2O dil to 200 ml; read in a Beckman spectrophotometer 1 cm cell).

Use

Biological stain.