2814. Dansyl Chloride

Nomenclature

CAS number: 605-65-2
5-(Dimethylamino)-1-naphthalenesulfonyl chloride.
C12H12ClNO2S; mol wt 269.75.
C 53.43%, H 4.48%, Cl 13.14%, N 5.19%, O 11.86%, S 11.89%.

Description and references

Reagent for fluorescent labelling of amines, amino acids and proteins. Synthesis: G. Weber, Biochem. J. 51, 155 (1952); A. Mendel, J. Chem. Eng. Data 15, 340 (1970). In amino acid analysis: J. Airhart et al., Anal. Biochem. 53, 132 (1973). In HPLC analysis of phenols: R. M. Cassidy, D. S. LeGay, J. Chromatogr. Sci. 12, 85 (1974). In protein microassays: T. Kinoshita et al., Anal. Biochem. 66, 104 (1975). In measurement of desquamation: R. Marks et al., Br. J. Dermatol. 111, 265 (1984).

Chemical structure

Properties

Yellow-orange crystals from hexane, mp 66.5-68°. Sol in acetone, pyridine, benzene, dioxane. Insol in water.

Use

In fluorescent labelling, column chromatography, HPLC.