3905. Evan's Blue

Nomenclature

CAS number: 314-13-6
6,6′-[(3,3′-Dimethyl[1,1′-biphenyl]-4,4′-diyl)bis(azo)]bis[4-amino-5-hydroxy-1,3-naphthalenedisulfonic acid] tetrasodium salt; C.I. Direct Blue 53; 4,4′-bis[7-(1-amino-8-hydroxy-2,4-disulfo)naphthylazo]-3,3′-bitolyl tetrasodium salt; C.I. 23860; T-1824; Azovan Blue.
C34H24N6Na4O14S4; mol wt 960.81.
C 42.50%, H 2.52%, N 8.75%, Na 9.57%, O 23.31%, S 13.35%.

Description and references

Prepd by coupling 1 mol of diazotized o-tolidine with 2 mols of Chicago acid (1-amino-8-naphthol-2,4-disulfonic acid): DE 35341; DE 38802 Frdl. 1, 469, 488 (1877-1887); DE 3949; DE 57327; DE 75469 Frdl. 3, 685, 687, 690 (1890-1894); Hartwell, Fieser, Org. Synth. coll. vol. II, 145 (1943). Diagnostic use: M. H. Nielsen, N. C. Nielsen, Scand. J. Clin. Lab. Invest. 14, 605 (1962); O. Linderkamp et al., Eur. J. Pediatr. 125, 135 (1977).

Chemical structure

Properties

Blue crystals with bronze to green luster. Sol in water, alcohol, acids, alkalies. Indicator changing color near pH 10. Destroyed by strong oxidizing and reducing agents and precipitated from soln by strong concns of neutral salts. Rather stable in aq soln, and may be autoclaved at 15 lbs pressure for 30 min. Dye made up in physiological saline should not be autoclaved.

Therapeutic Category

Diagnostic aid (blood volume determination).

Keywords

Diagnostic Aid