8042. Quin2

Nomenclature

CAS number: 73630-23-6 (salt); 83014-44-2 (acid)
N-[2-[[8-[Bis(carboxymethyl)amino]-6-methoxy-2-quinolinyl]methoxy]-4-methylphenyl]-N-(carboxymethyl)glycine tetrapotassium salt; 2-[[2-[bis(carboxymethyl)amino]-5-methylphenoxy]methyl]-6-methoxy-8-[bis(carboxymethyl)amino]quinoline; quin-2.
C26H23K4N3O10; mol wt 693.87.
C 45.01%, H 3.34%, K 22.54%, N 6.06%, O 23.06%.

Description and references

Fluorescent indicator dye which is primarily used for measuring changes in intracellular Ca2+. Increases in ion concn results in increased fluorescence without a shift in wavelengths. Prepn: R. Y. Tsien, Biochemistry 19, 2396 (1980). Use in measuring Ca2+ flow: J. N. Crofts, G. J. Barritt, Biochem. J. 264, 61 (1989); Zn2+-protein interactions: J. R. Jefferson et al., Anal. Biochem. 187, 328 (1990); in fluorescent lifetime imaging of cells: J. R. Lakowicz et al., Cell Calcium 15, 7 (1994). Fluorescent properties of acetoxymethyl ester: N. Miyoshi et al., Appl. Fluoresc. Technol. 4, 3 (1992). Dissociation parameters for Ca2+ and Mg2+ complexes: D. T. W. Bryant, Biochem. J. 226, 613 (1985); and photophysics: V. Van Den Bergh et al., Photochem. Photobiol. 61, 442 (1995). Review of use in measurement of Ca2+: R. Y. Tsien, T. Pozzan, Methods Enzymol. 172, 230-232 (1989).

Chemical structure

Properties

uv max absorption (0.1M KCl): 261, 354 nm (ε 37000, 5000) free dye; 240, 332 nm (ε 36000, 5000) Ca2+ complex.

Derivative

Acetoxymethyl ester.

Nomenclature

CAS number: 83104-85-2
Quin-2AM.
C38H43N3O18; mol wt 829.76.
C 55.00%, H 5.22%, N 5.06%, O 34.71%.

Use

Fluorescent probe for measurement of calcium in biological systems and for selected divalent cations.