9792. Trypan Blue

Nomenclature

CAS number: 72-57-1
3,3′-[(3,3′-Dimethyl[1,1′-biphenyl]-4,4′-diyl)bis(azo)]bis[5-amino-4-hydroxy-2,7-naphthalenedisulfonic acid] tetrasodium salt; C.I. Direct Blue 14; C.I. 23850; 3,3′-[(3,3′-dimethyl-4,4′-biphenylene)bis(azo)]bis(5-amino-4-hydroxy-2,7-naphthalenedisulfonic acid) tetrasodium salt; tetrasodium 3,3′-[(3,3′-dimethyl-4,4′-biphenylene)bis(azo)]bis(5-amino-4-hydroxy-2,7-naphthalenedisulfonate); sodium ditolyl-diazobis-8-amino-1-naphthol-3,6-disulfonate; Benzamine Blue; Diamine Blue; Benzo Blue; Congo Blue; Dianil Blue; Naphthylamine Blue; Niagara Blue.
C34H24N6Na4O14S4; mol wt 960.81.
C 42.50%, H 2.52%, N 8.75%, Na 9.57%, O 23.31%, S 13.35%.

Description and references

Prepd by coupling diazotized o-tolidine with 5-amino-4-hydroxy-2,7-naphthalenedisulfonic acid in sodium carbonate soln: Lewers, Lowy, Ind. Eng. Chem. 17, 1289-1290 (1925); Colour Index vol. 4 (3rd ed., 1971) p 4198. Toxicity study: Anderson et al., Proc. Soc. Exp. Biol. Med. 31, 825 (1934). Review of teratogenicity studies: R. L. Cahen, Clin. Pharmacol. Ther. 5, 480 (1964).

Chemical structure

Properties

Bluish-gray powder. Sol in water forming a deep blue soln with violet tinge. Almost insol in alcohol. LD100 i.v. in rats: 300 mg/kg (Anderson).

Use

Biological stain.