SDS-PAGE Running Buffer

Dissolve 151.4 g of tris(hydroxymethyl)aminomethane, 721.0 g of glycine and 50.0 g of sodium lauryl sulfate in water and dilute to 5000 mL with the same solvent. Immediately before use, dilute to 10 times its volume with water and mix. Measure the pH (2.2.3) of the diluted solution. The pH is between 8.1 and 8.8.