Silica Gel GF₂₅₄

(112926-00-8)

Contains about 13% of calcium sulfate hemihydrate and about 1.5% of a fluorescent indicator having an optimal intensity at 254 nm.

Fine, white or almost white, homogeneous powder with a particle size of about 15 μm.

Calcium sulfate content Determine by the method prescribed for silica gel G.

pH (2.2.3) Complies with the test prescribed for silica gel G.

Fluorescence Thin-layer chromatography (2.2.27) using silica gel GF₂₅₄ as the coating substance. Apply separately to the plate at ten points increasing volumes from 1 μL to 10 μL of a 1 g/L solution of benzoic acid in a mixture of 10 volumes of anhydrous formic acid and 90 volumes of 2-propanol. Develop over a path of 10 cm with the same mixture of solvents. After evaporating the solvents examine the chromatogram in ultraviolet light at 254 nm. The benzoic acid appears as dark spots on a fluorescent background in the upper third of the chromatogram for quantities of 2 μg and greater.