Tris-chloride Buffer pH 7.5

Tris(hydroxymethyl)aminomethane buffer solution pH 7.5.

Dissolve 7.27 g of tris(hydroxymethyl)aminomethane R and 5.27 g of sodium chloride R in water R, and adjust the pH (2.2.3) if necessary. Dilute to 1000.0 mL with water R.