Tris-EDTA BSA Buffer Solution pH 8.4

Dissolve 6.1 g of tris(hydroxymethyl)aminomethane R, 2.8 g of sodium edetate R, 10.2 g of sodium chloride R and 10 g of bovine albumin R in water R, adjust to pH 8.4 (2.2.3) using 1 M hydrochloric acid and dilute to 1000.0 mL with water R.