Hydroflumethiazide Tablets
Action and use
Thiazide diuretic.
Definition
Hydroflumethiazide Tablets contain Hydroflumethiazide.
Content of hydroflumethiazide, C8H8F3N3O4S2
92.5 to 107.5% of the stated amount.
Identification
Carry out the method for thin-layer chromatography, Appendix III A, using the following solutions in acetone.
By each method of visualisation the principal spot in the chromatogram obtained with solution (1) corresponds in colour and intensity to that in the chromatogram obtained with solution (2).
TEST
Related substances
Carry out the method for thin-layer chromatography, Appendix III A, using the following solutions in acetone.
Any secondary spot in the chromatogram obtained with solution (1) is not more intense than the spot in the chromatogram obtained with solution (2) (1%).
Assay
Weigh and powder 20 tablets. Shake a quantity of the powder containing 15 mg of Hydroflumethiazide with 50 mL of methanol for 10 minutes and dilute to 100 mL with methanol. Mix, filter, dilute 10 mL of the filtrate to 100 mL with methanol and measure the absorbance of the resulting solution at the maximum at 273 nm, Appendix II B. Calculate the content of C8H8F3N3O4S2 taking 595 as the value of A(1%, 1 cm) at the maximum at 273 nm.