Nicotinamide Tablets
Action and Use
Component of vitamin B.
Definition
Nicotinamide Tablets contain Nicotinamide.
Content of nicotinamide, C6H6N2O
92.5 to 107.5% of the stated amount.
Identification
Tests
Related substances
Carry out the method for thin-layer chromatography, Appendix III A, using the following solutions.
10 volumes of water, 45 volumes of ethanol (96%) and 48 volumes of chloroform.
Any secondary spot in the chromatogram obtained with solution (1) is not more intense than the spot in the chromatogram obtained with solution (2) (0.25%).
Assay
Weigh and powder 20 tablets. Shake a quantity of the powder containing 50 mg of Nicotinamide with 50 mL of ethanol (96%), dilute to 100 mL with ethanol (96%) and filter. Dilute 5 mL of the filtrate to 100 mL with ethanol (96%) and measure the absorbance of the resulting solution at the maximum at 262 nm, Appendix II B. Calculate the content of C6H6N2O taking 241 as the value of A(1%, 1 cm) at the maximum at 262 nm.
Storage
Nicotinamide Tablets should be protected from light.