Strong Glutaraldehyde Solution

General Notices

C5H8O2 100.1 111-30-8 
  (anhydrous) 

Action and use

Used in treatment of warts.

Preparation

Glutaraldehyde Solution

Definition

Strong Glutaraldehyde Solution is an aqueous solution of glutaraldehyde (pentanedial). It contains not less than 47.0% and not more than 53.0% w/w of glutaraldehyde, C5H8O2.

Characteristics

A colourless or almost colourless solution.

Identification

A. Heat 1 mL with 10 mL of a solution containing 1 g of hydroxylamine hydrochloride and 2 g of sodium acetate in water on a water bath for 10 minutes, allow to cool and filter. The melting point of the residue, after washing with water and drying at 105°, is about 178°, Appendix V A.
B. Add 0.05 mL to 2 mL of ammoniacal silver nitrate solution and mix gently for a few minutes. Silver is deposited.

Tests

Acidity

Dilute 10 mL with 10 mL of carbon dioxide-free water and titrate with 0.1m sodium hydroxide VS using bromothymol blue solution R3 as indicator. Not more than 5.0 mL of 0.1m sodium hydroxide VS is required to change the colour of the solution.

Clarity and colour of solution

Dilute 1 volume with 4 volumes of water. The resulting solution is clear, Appendix IV A, and not more intensely coloured than reference solution B6, Appendix IV B, Method I.

Weight per mL

1.126 to 1.134 g, Appendix V G.

Assay

Dissolve 4 g in 100 mL of a 7% w/v solution of hydroxylamine hydrochloride previously neutralised to bromophenol blue solution with 1m sodium hydroxide VS and allow to stand for 30 minutes. Add 20 mL of petroleum spirit (boiling range, 40° to 60°) and titrate with 1m sodium hydroxide VS until the colour of the aqueous phase matches that of a 7% w/v solution of hydroxylamine hydrochloride previously neutralised to bromophenol blue solution with 1m sodium hydroxide VS. Each mL of 1m sodium hydroxide VS is equivalent to 50.05 mg of C5H8O2.

Storage

Strong Glutaraldehyde Solution should be stored at a temperature not exceeding 15°.