7479. PIPES

Nomenclature

CAS number: 5625-37-6
1,4-Piperazinediethanesulfonic acid; piperazine-N,N′-bis(2-ethanesulfonic acid); 1,4-piperazinebis(ethanesulfonic acid).
C8H18N2O6S2; mol wt 302.37.
C 31.78%, H 6.00%, N 9.26%, O 31.75%, S 21.21%.

Description and references

One of the zwitterionic N-substituted aminosulfonic acids known as “Good” buffers; active in the pH range 6-8.5. Prepn: N. Good et al., Biochemistry 5, 467 (1966). Interference with Lowry protein determn: H. M. Himmel, W. Heller, J. Clin. Chem. Clin. Biochem. 25, 909 (1987). Use as biological buffer: R. Salema, I. Brandao, J. Submicrosc. Cytol. 5, 79 (1973); S. Haviernick et al., J. Microsc. 135, 83 (1984).

Chemical structure

Derivative

Monosodium salt.

Nomenclature

CAS number: 10010-67-0
C8H17N2NaO6S2; mol wt 324.35.
C 29.62%, H 5.28%, N 8.64%, Na 7.09%, O 29.60%, S 19.77%.

Properties

Crystals from water and alcohol, mp >300° (dec). pKa1 ≈3, pKa2 (20°): 6.82 (0.1M); 6.82 (0.2M); 6.96 (0.01M). ΔpKa/°C -0.0085.

Derivative

Disodium salt.

Nomenclature

CAS number: 76836-02-7
Sodium pipesate.
C8H16N2Na2O6S2; mol wt 346.33.
C 27.74%, H 4.66%, N 8.09%, Na 13.28%, O 27.72%, S 18.52%.

Use

Biological buffer.