9795. Trypsin

Nomenclature

CAS number: 9002-07-7
Parenzymol (Horner); Trypure (Novo).

Description and references

Mol wt 24,000. Proteolytic enzyme formed in the small intestine by the action of a peptidase, enterokinase, on the pancreatic cell product, trypsinogen. Acts on lysyl and arginyl bonds of peptide chains and hydrolyzes even esters and amides. Reviews: Desnuelle, “Trypsin” in The Enzymes vol. 4, P. D. Boyer et al., Eds. (Academic Press, New York, 2nd ed., 1960) pp 119-132; Keil, ibid. vol. 3 (3rd ed., 1971) pp 250-275; Inagami, “Trypsin” in Proteins, Structure and Function vol. 1, M. Funatsu et al., Eds. (Kodansha, Tokyo, Wiley, New York, 1972) pp 1-83.

Properties

Yellow to grayish-yellow powder or crystals. Stable indefinitely in dry form at room temp. Sol in water. Practically insol in alcohol or glycerol. Readily sol in Sorensen's sodium phosphate buffer soln. Acts optimally at pH values between 7 and 9. Solns lose 75% of their potency within 3 hrs at room temp. Prepn of stabilized trypsin compositions contg partially hydrolyzed gelatin: Sullivan, Martin, US 2930736 (1960 to National Drug).

Therapeutic Category

Enzyme (proteolytic).

Therapeutic Category (Veterinary)

Enzyme (proteolytic).

Keywords

Enzyme; Proteolytic