Bendroflumethiazide Tablets
Action and use
Thiazide diuretic.
Definition
Bendroflumethiazide Tablets contain Bendroflumethiazide.
Content of bendroflumethiazide, C15H14F3N3O4S2
92.5 to 107.5% of the stated amount.
Identification
Carry out the method for thin-layer chromatography, Appendix III A, using the following solutions.
By each method of visualisation the principal spot in the chromatogram obtained with solution (1) corresponds in position and colour to that in the chromatogram obtained with solution (2).
TESTS
Related substances
Carry out the method for thin-layer chromatography, Appendix III A, using the following solutions.
Any secondary spot in the chromatogram obtained with solution (1) is not more intense than the spot in the chromatogram obtained with solution (2) (1.0%).
Assay
Weigh and powder 20 tablets. Mix a quantity of the powder containing 15 mg of Bendroflumethiazide with 50 mL of 0.1m sodium hydroxide with the aid of ultrasound for 10 minutes and dilute to 100 mL with 0.1m sodium hydroxide. Mix, filter, dilute 10 mL of the filtrate to 100 mL with water and measure the absorbance of the resulting solution at the maximum at 275 nm, Appendix II B. Calculate the content of C15H14F3N3O4S2 taking 410 as the value of A(1%, 1 cm) at the maximum at 275 nm.