Acetazolamide Tablets
Action and use
Carbonic anhydrase inhibitor; diuretic; treatment of glaucoma, ocular hypertension, mountain sickness.
Definition
Acetazolamide Tablets contain Acetazolamide.
Content of acetazolamide, C4H6N4O3S2
95.0 to 105.0% of the stated amount.
Identification
TEST
Related substances
Carry out the method for thin-layer chromatography, Appendix III A, using the following solutions.
20 volumes of 13.5m ammonia, 30 volumes of ethyl acetate and 50 volumes of propan-2-ol.
Any secondary spot in the chromatogram obtained with solution (1) is not more intense than the spot in the chromatogram obtained with solution (2) (1%).
Assay
Weigh and powder 20 tablets. To a quantity of the powder containing 0.4 g of Acetazolamide add 90 mL of dimethylformamide and carry out Method II for non-aqueous titration, Appendix VIII A, using 0.1m tetrabutylammonium hydroxide VS as titrant and determining the end point potentiometrically. Each mL of 0.1m tetrabutylammonium hydroxide VS is equivalent to 22.22 mg of C4H6N4O3S2.